TO BE RESCINDED
901-15-03 Services performed by the division of plant health.
(A)Samples for seed testing shall be tested in order of receipt during the period ofJanuary first through May thirty-first of each year and charges made in accordancewith the fee schedules contained in paragraphs (C), (D) and (E) of this rule.(B)Samples for seed testing shall be tested for purity and germination, when requestedand in order of receipt, without cost during the period of June first throughDecember thirty-first of each year in accordance with the following schedule:(1)Three samples of seed of any kind, excluding mixes of seed; or(2)One sample of seed that is a mixed seed sample.(C)The fee for purity testing performed in accordance with rule 901-15-01 of theAdministrative Code for each sample submitted exceeding the limitation inparagraph (B) of this rule shall be:(1)For cereals, including barley, oats, rye, wheat and similar species: nine dollarsand fifty cents;(2)For all vegetables: ten dollars and fifty cents;(3)For legumes and forages, including alfalfa, clovers, millet, trefoil, rape,lespedeza, sudangrass, vetch and similar species: twelve dollars and fiftycents;(4)For the following grasses: timothy, brome and redtop: eighteen dollars;(5)For grasses other than timothy, brome and redtop: twenty dollars. Thefluorescence test for ryegrass is an additional fee of four dollars and fiftycents;(6)For corn and soybeans: twelve dollars and fifty cents; and(7)For flowers: ten dollars.(D)The fee for germination testing performed in accordance with rule 901-15-01 of theAdministrative Code for each sample submitted exceeding the limitation inparagraph (B) of this rule shall be:(1)For cereals, including barley, oats, rye, wheat and similar species: seven dollarsand fifty cents;(2)For the following vegetables: beets, spinach, carrots, parsnips, asparagus andparsley: eleven dollars and fifty cents;(3)For vegetables other than beets, spinach, carrots, parsnips, asparagus andparsley: ten dollars.(4)For legumes and forages, including alfalfa, clovers, millet, trefoil, rape,lespedeza, sudangrass, vetch and similar species: eight dollars and fifty cents;(5)For the following grasses: timothy, brome and redtop: twelve dollars;(6)For grasses other than timothy, brome and redtop: fourteen dollars and fiftycents;(7)For corn and soybeans: nine dollars; and(8)For flowers: twelve dollars.(E)The fee for the following services performed in accordance with rule 901-15-01 ofthe Administrative Code shall be, for each sample submitted:(1)For a cold test: seventeen dollars;(2)For an Ohio noxious weed seed examination: half of the purity testing fee forthat particular type of seed as established in paragraph (C) of this rule . If thesample is for a seed mixture, the fee shall be half of the purity testing fee forthe seed forming the largest component of the mixture by weight;(3)For germination-only testing, the separation fee per component of a mixture:five dollars;(4)For excessive contamination with weed or other crop seed: three dollars andfifty cents for each whole per cent, or part thereof that the contaminationexceeds one per cent of the total amount of the seed sample, by weight; and(5)For tetrazolium testing: twenty dollars.(F)The fee for the following services, conducted as part of the Ohio plant diagnosticnetwork, shall be:(1)Base fee: a fee of up to thirty dollars will be charged for homeowner submittedsamples. The base fee includes microscopic examination of sample and moistchambering/basic culturing on media.(2)Soybean cyst nematode (SCN) test: A fee of up to twenty-five dollars will becharged for processing a soil sample for SCN. The fee includes cyst and/oregg counts.(3)Non-SCN nematode test: a fee of up to thirty dollars will be charged per tissueor soil sample. Fee includes extraction by Baermann funnel or pie platemethod, and nematode count.(4)Enzyme-linked immunosorbent assay (ELISA) for common pathogens: a fee ofup to fifty dollars will be charged per tissue sample. Fee includes samplepreparation, sample test in duplicate wells, and spectrophotometric evaluationof test wells. Fee is applicable to common pathogens and does not apply tomycotoxin or pesticide ELISA tests.(5)Quantitative ELISA for mycotoxins: a fee of up to sixty dollars will be chargedper grain or tissue samples. Fee includes sample preparation, sample test insingle test well plus known standards, and spectrophotmetric evaluation oftest wells.(6)Fatty acid methyl esterase (FAME)/ Gas chromatography (GC): a fee of up tosixty dollars will be charged per sample. Fee includes sample preparation andGC analysis.(7)Double-stranded ribonucleic acid (dsRNA) analysis: a fee of up to sixty dollarswill be charged per sample. Fee includes dsRNA extraction, purification, andelectrophoretic analysis.(8)Polymerase chain reaction (PCR): a fee of up to sixty dollars will be chargedper sample. Fee includes DNA extraction, purification, PCR reaction, andelectrophoretic analysis.(9)Immunocapture reverse-transcription PCR (IC-RT-PCR): a fee of up to seventydollars will be charged per sample. Fee includes immunocapture withantibody-conjugated magnetic beads, cDNA synthesis by reversetranscription, PCR reaction, and electrophoretic analysis.(10)RT-PCR using cDNA from dsRNA or total RNA template: a fee of up to onehundred twenty-five dollars will be charged per sample. Fee includesdsRNA/total RNA extraction/purification, cDNA synthesis by reversetranscription, PCR reaction, and electrophoretic analysis.(11)Unknown fungi ITS real time PCR assay: a fee of up to eighty dollars will becharged per sample. Fee includes DNA extraction, ITS region amplificationby real time PCR, DNA sequencing, and sequence results subjected to aBLASTn database search.(12)Real time PCR for detection of specific pathogens: a fee of up to seventy-fivedollars will be charged per sample.(13)Cloning and sequencing PCR products: a fee of up to one hundred dollars willbe charged per PCR product to be cloned, squencing will be an additional feeof up to ten dollars per reaction. Fee includes amplification using specificprimers, DNA purification, PCR products ligated into a plasmid vector,transformation of E. coli cells, and screening clones for insert by PCR.(14)Virus detection/identification by partial purification and transmission electronmicroscopy (TEM): a fee of up eighty dollars will be charged per sample. Feeincludes partial virus purification from plant tissue, negative stainingpreparation, and TEM evaluation.Effective: 01/14/2016
Five Year Review (FYR) Dates: 10/30/2015
CERTIFIED ELECTRONICALLY
Certification
01/04/2016
Date
Promulgated Under: 119.03
Statutory Authority: 901.43, 907.10
Rule Amplifies: 901.43, 907.10
Prior Effective Dates: 10/24/80, 10/31/04
Document Information
- Effective Date:
- 1/14/2016
- File Date:
- 2016-01-04
- Last Day in Effect:
- 2016-01-14
- Five Year Review:
- Yes
- Rule File:
- 901-15-03_PH_FF_R_RU_20160104_0807.pdf
- Related Chapter/Rule NO.: (1)
- Ill. Adm. Code 901-15-03. Services performed by the division of plant health